Amphibian Conservation

Chen, D.M., Moore, M.G., Willis, E.L., Kouba, A.J. and Kouba, C.K. (2022). The impact of time and environmental factors on the mitochondrial vesicle and subsequent motility of amphibian sperm. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 268, p.111191. https://doi.org/10.1016/j.cbpa.2022.111191

Julien, A.R., Counsell, K.R., Burger, I.J., Kouba, A.J., Barber, D., Cobos, C., Jennings, R.D., Christman, B.L. and Kouba, C.K. (2022). Effects of wild, semi-captive, and captive management on male Chiricahua leopard frog sperm quality with implications for conservation breeding programs. Conservation Science and Practice, p.e12864. https://doi.org/10.1111/csp2.12864

Arregui, L., Kouba, A.J., Germano, J.M., Barrios, L., Moore, M., Kouba, C.K. (2021). Fertilization potential of cold-stored Fowler's toad (Anaxyrus fowleri) spermatozoa: temporal changes in sperm motility based on temperature and osmolality. Reproduction, Fertility and Development, 34(5), pp.461-469. https://doi.org/10.1071/RD21037

Bronson, E., Guy, E.L., Murphy, K.J., Barrett, K., Kouba, A.J., Poole, V. and Kouba, C.K. (2021). Influence of oviposition-inducing hormone on spawning and mortality in the endangered Panamanian golden frog (Atelopus zeteki). BMC Zoology, 6(1), pp.1-14. https://doi.org/10.1186/s40850-021-00076-8

Gillis, A.B., Guy, E.L., Kouba, A.J., Allen P.J., Marcec-Greaves, R.M., Kouba, C.K. (2021). Short-term storage of tiger salamander (Ambystoma tigrinum) spermatozoa: the effect of collection type, temperature, and time. PLoS ONE 16(1): e0245047. https://doi.org/10.1371/journal.pone.0245047

Kouba, A.J., Langhorne, C.J., Willard, S.T., Smith, T., Kouba, C.K. (2021). Spermiation response to exogenous hormone therapy in hibernated and non-hibernated boreal toads (Anaxyrus boreas boreas). Reproduction, Fertility and Development, 34(5), pp.453-460. https://doi.org/10.1071/RD21033

Langhorne, C., Willard, S.T., Vance, C.K., Calatayud, N., Smith, T., Ryan, P., Kouba, A.J. (2021). Efficacy of hormone stimulation on sperm collection in an alpine amphibian (Anaxyrus boreas boreas) and the effect of short-term storage on sperm quality. Zoology 146, p.125912. https://doi.org/10.1016/j.zool.2021.125912

Arregui, L., Bóveda, P., Gosálvez, J. and Kouba, A.J (2020). Effect of seasonality on hormonally induced sperm in Epidalea calamita (Amphibia, Anura, Bufonidae) and its refrigerated and cryopreservated storage. Aquaculture, 529, p.735677. https://doi.org/10.1016/j.aquaculture.2020.735677

Guy, E.L., Martin, M.W., Kouba, A.J., Cole, J.A., Kouba, C.K. (2020). Evaluation of different temporal periods between hormone-induced ovulation attempts in the female Fowler's toad (Anaxyrus Fowleri). Conservation Physiology, Volume 8, Issue 1. https://doi.org/10.1093/conphys/coz113

Arregui, L., Diaz-Diaz, S., Alonso-Lopez, E., Kouba, A.J. (2019). Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita). Reproductive Biology and Endocrinology Journal, 17:92 https://doi.org/10.1186/s12958-019-0537-0

Graham, G.M., Langhorne, C.J., Vance, C.K., Willard, S.T., Kouba, A.J. (2019). Ultrasound imaging improves hormone therapy strategies for induction of ovulation and in vitro fertilization in the endangered dusky gopher frog (Lithobates sevosa). Conservation Physiology, Volume 6: Issue 1. https://doi.org/10.1093/conphys/coy020

Julien, A.R., Kouba, A.J., Kabelik, D., Feugang, J.NM., Willard, S.T., Kouba, C.K. (2019). Nasal administration of gonadotropin releasing hormone (GnRH) elicits sperm production in Fowler’s toads (Anaxyrus fowleri). BMC Zoology, 4:3. https://doi.org/10.1186/s40850-019-0040-2

Earl, J.E., Hill, R.D., Chaney, J.C., Lillard, C.E., Sutton, W. B., Kouba, A.J., Langhorne, C., Krebs, J., Wilkes, R., Miller, D.L., Gray, M.J. (2016). Ranavirus could facilitate local extinctions of rare amphibian species. Oecologia, 182 (2): 611-623. https://doi.org /10.1007/s00442-016-3682-6

Graham, K., Kouba, A.J., Langhorne, C.J., Marcec, R.M., Willard, T. (2016). Biological sex identification in the endangered dusky gopher frog (Lithobates sevosa): a comparison of body size measurements, secondary sex characteristics, ultrasound imaging and urinary hormone analysis methods. Journal Reproductive Biology and Endocrinology, 14:41. https://doi.org /10.1186/s12958-016-0174-9

McDonough, C., Martin, M., Vance, C., Cole, J., Kouba, A. (2016). Frequency of exogenous hormone therapy impacts spermiation in male Fowler toads (Bufo fowleri). Reproduction, Fertility and Development, 28 (7): 995-1003. https://doi.org/10.1071/RD14214

Calatayud, N.E., Langhorne, C.J., Mullen, A.K., Williams, C.L., Smith, T., Bullock, L., Kouba, A.J., Willard, S. (2015). A Hormone priming regimen and hibernation affect spontaneous oviposition in the boreal toad (Anaxyrus boreas boreas). Theriogenology, 84 (4): 600-607 https://doi.org/10.1016/j.theriogenology.2015.04.017

Sutton, W.B., Gray, M.J., Hardman, R.H., Wilkes, R.P., Kouba, A.J., Miller, D.L. (2014). High Susceptibility of the Endangered Dusky Gopher Frog to Ranavirus. Diseases of Aquatic Organisms, 112: 9-16. https://doi.org/10.3354/dao02792

Germano, J., Arregui, L., Kouba, A.J. (2013). Effect of aeration and antibiotics on short term storage of Fowler's toad (Bufo fowleri) sperm. Aquaculture, 396-399: 20-24. https://doi.org/10.1016/j.aquaculture.2013.02.018

Davis, J.R., Eastlack, D.T., Kouba, A.J., Vance, C.K. (2012). Batrachochytrium dendrobatidis detected in Fowler's toad (Anaxyrus fowleri) populations in Memphis, Tennessee, USA. Herpetological Review, 43: 81-83.

Kouba, A.J, Trillo, D.B., Vance, C.K., Milam, C., Carr, M. (2012). A comparison of human chorionic gonadotropin and luteinizing hormone releasing hormone on the induction of spermiation and amplexus in the American toad (Anaxyrus americanus). Journal of Reproductive Biology and Endocrinology, 10:59. https://doi.org/10.1186/1477-7827-10-59

Kouba, A.J., Vance, C.K., Willis, E. (2009). Artificial fertilization for the conservation of amphibians: Current knowledge and future considerations. Theriogenology, 71: 214-227. https://doi.org/10.1016/j.theriogenology.2008.09.055

Li, H., Kouba, A.J., Seratt, J.L., Vaughan, M.J., Browne, R.K. (2009). A complex enrichment diet improves growth and health in the endangered Wyoming toad (Bufo baxteri). Zoo Biology, 28:197-213

Browne, R.K., Li, H., Seratt, J., Kouba, A.J. (2006). Progesterone improves the number and quality of hormone induced Fowler toad (Bufo fowleri) oocytes. Journal of Reproductive Biology and Endocrinology. 2006, 4:3. https://doi.org/10.1186/1477-7827-4-3

Browne, R.K., Seratt, J., Vance, C.K., Kouba, A.J. (2006). Hormonal priming, induction of ovulation and in-vitro fertilization of the endangered Wyoming toad (Bufo baxteri). Journal of Reproductive Biology and Endocrinology, 4:34. https://doi.org/10.1186/1477-7827-4-34

Kouba, A.J., Vance, C.K., Frommeyer, M.A., Roth T.L. (2003). Structural and functional aspects of Bufo americanus spermatozoa: Effects of inactivation and reactivation. Journal of Experimental Zoology. 295A: 172-182. https://doi.org/10.1002/jez.a.10192